表达区间及表达系统(Source)
Mouse Clec2e Protein, Fc Tag (CLE-M5253) is expressed from human 293 cells (HEK293). It contains AA Val 60 - Gly 204 (Accession # Q80XD9-1).
Predicted N-terminus: Val 60
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蛋白结构(Molecular Characterization)
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 43.0 kDa. The protein migrates as 53-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Mouse Clec2e Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse Clec2e Protein, Fc Tag (Cat. No. CLE-M5253) is more than 85% and the molecular weight of this protein is around 90-110 kDa verified by SEC-MALS.
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背景(Background)
CLEC2e (C-type lectin domain family 2 member e) is also known as Clra, clr-a. Clec2e is a receptor for lectin-type cell surface.