膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-AAV5 Antibody, Mouse Fc (10B4E6) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
克隆号（Clone）
10B4E6
种属（Species）
Mouse
亚型（Isotype）
Mouse Fc
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Hybridoma Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Adeno-Associated Virus (AAV) 5.
特异性（Specificity）
This product is a specific antibody specifically reacts with AAV5.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.2-100 ng/mL |
纯度（Purity）
>90% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-AAV5 Antibody, Mouse Fc (10B4E6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized AVV5 at 4.35E+10 capsids/mL (100 μL/well) can bind Monoclonal Anti-AAV5 Antibody, Mouse Fc (10B4E6) (Cat. No.AA5-Y188) with a linear range of 0.20-3.13 ng/mL (QC tested).
Protocol
背景（Background）
The adeno-associated virus (AAV) is a small (25 nm), non-enveloped virus of the parvoviridae family, including 12 different AAV serotypes. In the parvoviridae family it belongs to the genus dependoparvovirus, because it needs the presence of a helper virus for replication and assembly. The icosahedral AAV capsid composed of the capsid proteins VP1, VP2 and VP3 contains a linear, single-stranded DNA genome of 4.7 kb.
