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Monoclonal Anti-SN38 Antibody, Mouse IgG1 (MALS verified)

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货号-规格
价格
Qty.
SN8-M685-100ug
¥6280.00
SN8-M685-1mg
¥41790.00
合计0件 产品金额¥ 0

产品详情

  • 特异性(Specificity)

    Specifically recognizes the target-SN38.

  • 抗体来源(Source)

    Monoclonal Anti-SN38 Antibody, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • 亚型(Isotype)

    Mouse IgG1 | Mouse Kappa

  • 偶联(Conjugate)

    Unconjugated

  • 种属反应性(Reactivity)

    Chemical

  • 免疫原(Immunogen)

    SN38-OVA.

  • 应用(Application)

    Application
    Recommended Usage
    ELISA
    0.1-8 ng/mL 
  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 活性(Bioactivity)-ELISA

     SN38 ELISA

    Immobilized Sacituzumab govitecam at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) with a linear range of 0.12-0.98 ng/mL (QC tested).

    Protocol
  •  SN38 ELISA

    Immobilized Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) at 1 μg/mL, add Sacituzumab Govitecam in the 50% Mouse serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).

    Protocol
  • 交叉验证-Cross Verification

     SN38 CROSS_VERIFICATION

    ELISA binding of Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) with Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibody respectively.

    The coating antibody was Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and Trastuzumab-DM1 conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was Peroxidase AffiniPure Goat Anti-Human IgG, Fcγ fragment specific (min X Bov, Hrs, Ms Sr Prot) (Jackson, Cat. No. 109-035-098) used at 1:12000 concentration.

    Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-M685) is specific to Sacituzumab Govitecam and has no cross-reactivity with Trastuzumab Deruxtecan, Trastuzumab MMAE and Trastuzumab-DM1 (Routinely tested).

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背景介绍

The 7-ethyl-10-hydroxycamptothecin (SN-38) is a topoisomerase I inhibitor (chemotherapeutic agent) used in cancer therapy. In a comparative study of SN-38 with a potent chemotherapeutic prodrug, irinotecan (CPT-11), SN-38 was found to be 1000-fold more cytotoxic against colorectal cancer cells as compared to irinotecan.

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