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分子别名（Synonym）
TNFRSF9,4-1BB,CD137,CDw137,ILA
表达区间及表达系统（Source）
Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag (41B-C82E9) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # A9YYE7-1).
Predicted N-terminus: Leu 24
Request for sequence蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 21.8 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag (Cat. No. 41B-C82E9) is more than 90% and the molecular weight of this protein is around 27-41 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag (Cat. No. 41B-C82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cynomolgus 4-1BB Ligand, Fc Tag (Cat. No. 41L-C5254) with a linear range of 0.3-2 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus 4-1BB Ligand, Fc Tag (Cat. No. 41L-C5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag (Cat. No. 41B-C82E9) with a linear range of 0.3-20 ng/mL (Routinely tested).
Protocol
Immobilized Human 4-1BB Ligand, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag (Cat. No. 41B-C82E9) with a linear range of 0.6-20 ng/mL (Routinely tested).
Protocol
Immobilized Utomilumab at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag (Cat. No. 41B-C82E9) with a linear range of 0.3-10 ng/mL (Routinely tested).
Protocol
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