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分子别名（Synonym）
FCGR
表达区间及表达系统（Source）
Mouse CD32b, His Tag (CDB-M52H7) is expressed from human 293 cells (HEK293). It contains AA Thr 40 - Arg 217 (Accession # NP_001070657.1).
Predicted N-terminus: Thr 40
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.2 kDa. The protein migrates as 32-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Mouse CD32b, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse CD32b, His Tag (Cat. No. CDB-M52H7) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
OKT3 immobilized on CM5 Chip can bind Mouse CD32b, His Tag (Cat. No. CDB-M52H7) with an affinity constant of 498 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Immobilized OKT3 on Protein G-Series S sensor chip, can bind Mouse CD32b, His Tag (Cat. No. CDB-M52H7) with an affinity constant of 0.55 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Mouse CD32b, His Tag (Cat. No. CDB-M52H7) on NTA Biosensor, can bind OKT3 with an affinity constant of 1.2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Mouse CD32b, His Tag (Cat. No. CDB-M52H7) on NTA Biosensor, can bind Herceptin with an affinity constant of 2.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded OKT3 on Protein G Biosensor, can bind Mouse CD32b, His Tag (Cat. No. CDB-M52H7) with an affinity constant of 1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Herceptin on Protein G Biosensor, can bind Mouse CD32b, His Tag (Cat. No. CDB-M52H7) with an affinity constant of 1.2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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背景介绍
There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
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