产品详情
分子别名(Synonym)
INSR,Insulin receptor,IR,CD220
表达区间及表达系统(Source)
Biotinylated Human Insulin R (28-944) Protein, His,Avitag (INR-H82E6) is expressed from human 293 cells (HEK293). It contains AA His 28 - Lys 944 (Accession # P06213-2).
Predicted N-terminus: His 28
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 108.2 kDa. The protein migrates as 115-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
数据展示
电泳(SDS-PAGE)
Biotinylated Human Insulin R (28-944) Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human Insulin R (28-944) Protein, His,Avitag (Cat. No. INR-H82E6) is more than 80% and the molecular weight of this protein is around 275-315 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-SPR
Biotinylated Human Insulin R (28-944) Protein, His,Avitag (Cat. No. INR-H82E6) immobilized on SA Chip can bind Insulin with an affinity constant of 35.6 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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