ACROBiosystems百普赛斯Star Staining新一代定点标记技术平台开发的高质量荧光标记蛋白，可保持蛋白的天然构象，不影响蛋白活性，均一性好、批间一致性高。“星级”的研发实力，“星级”的技术平台及产品，Star Staining专为CAR-T细胞质控和临床样本分析检测而设计。
对于CAR-T细胞来说，发挥肿瘤杀伤作用的有效成分是CAR阳性的T细胞，CAR-T细胞产品的包装规格及临床使用剂量是以CAR-T阳性细胞数表示的，因此，CAR转染阳性率是CAR-T细胞产品质量控制的必检项，监管部门建议应采用流式细胞法（FACS）检测CAR转染阳性率。荧光标记蛋白因其预先标记了荧光素基团，可通过一步染色完成检测，既简化了检测流程、节省了检测时间，又避免了因使用二抗产生的非特异背景，成为流式检测CAR表达的首选试剂。
然而，荧光标记蛋白的选择面临着诸多难题，比如使用传统随机化标技术导致标记后蛋白活性下降、荧光强度弱而无法检测低丰度表达的CAR、流式检测设备局限于可选择的荧光类型等问题。
ACROBiosystems凭借Star Staining新一代定点标记技术平台，成功开发了一系列荧光标记类型的CAR靶点相关蛋白产品，以“星级”的产品优势，全方位满足CAR-T阳性率检测的不同需求。
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蛋白特定位点标记
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标记速度快效率高
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标记位点远离活性中心，维持蛋白天然空间构象
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高效生物正交反应，无非特异信号
经MALS验证，Alexa Fluor 555-Labeled Human BCMA Star Staining产品纯度高于95%。
The purity of Alexa Fluor 555-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining (Cat. No. BCA-HA2H6) is more than 95% and the molecular weight of this protein is around 24-36 kDa verified by SEC-MALS.
Star Staining PE-CD19 和APC-CD19蛋白产品可高灵敏检测CD19 CAR表达。
Anti-CD19 CAR-293 cells were stained with PE-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HP2H5), His Tag star staining and negative control protein respectively, PE signal was used to evaluate the binding activity.
Anti-CD19 CAR-293 cells were stained with APC-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HA2H9), His Tag star staining and negative control protein respectively, APC signal was used to evaluate the binding activity.
Star Staining FITC-BCMA和PE-BCMA蛋白产品的检测灵敏度显著高于竞品。
Binding activity of FITC-Labeled Human BCMA and PE-Labeled Human BCMA protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) have a much higher binding activity than that of the other competitor.
Star Staining FITC-BCMA和PE-BCMA产品经未转染CAR的PBMC细胞双染批检测验证，无非特异背景问题，特异性显著高于竞品，保证CAR表达检测结果的特异性和准确性，适用于CAR-T临床样本分析。
Non-specific binding to non-transduced PBMCs between PE-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with PE -Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and PE signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.
Non-specific binding to non-transduced PBMCs between FITC -Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with FITC -Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and FITC signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.
Star Staining FITC-labeled Human BCMA产品可保持天然蛋白活性。
Binding activity of the Human BCMA before and after FITC labeling was evaluated in the above FACS analysis. The result shows that FITC-Labeled BCMA (Cat. No. BCA-HF2H3) and unconjugated Human BCMA have almost the same level of binding activity.
Binding activity of different lots of AF555-Labeled Human BCMA (Cat. No. BCA-HA2H6) and PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) against anti-BCMA CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.
FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) at 25℃ for 48 hours and freeze-throw cycles are stable without performance reduction.
Star Ribbon预染蛋白Marker蛋白质标记物是生物研究和药物开发的重要组成部分。无论是用于蛋白质电泳还是western blot，我们的预染色蛋白质标记物帮助您快速确定目标蛋白质的分子量或评估转移效率。Fc受体蛋白治疗性抗体的功效取决于Fab片段及其对目标抗原的结合活性，还取决于Fc片段及其与关键Fc受体的相互作用。因此，在抗体工程中候选物必须针对一系列受体进行测试。探索我们的重组Fc受体蛋白质的全面收藏！
膜杰作
Star Staining
