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Fc受体蛋白

高纯度Fc受体蛋白
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背景
治疗性抗体的功效不仅取决于其Fab片段结合靶抗原的活性，还取决于其Fc片段与Fc受体的相互作用。Fc片段与FcRn（FCGRT&B2M）受体的亲和力可以预测抗体的半衰期，而Fc片段和FcγRⅢA（CD16a）的结合会影响抗体依赖的细胞介导的细胞毒性作用（Antibody-Dependent Cell-mediated Cytotoxicity）。因此，优化抗体结构，筛选获得和Fc受体亲和力最理想的抗体，是发展治疗性抗体药物的一个重要途径。
ACROBiosystems公司提供一系列Fc受体产品，包括了FcRn、FcγR及其常见的突变体，以帮助加速抗体药物的开发。 同时，基于我司丰富的蛋白产品资源和活性分析经验，您只需提供样品，我们即可为您提供优质SPR&BLI亲和力检测服务，且免费提供实验所需所有高质量Fc受体蛋白！（Fc受体蛋白作为分析物时除外） 点击快速咨询 >>
新品推荐：Human Fc gamma RI / CD64 Binding Kit（TR-FRET）， Human FcRn binding Kit (TR-FRET) 现在均已上线，操作简便，灵敏度高，检测快速，结果精准，助力您的药物研发快人一步。
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Fc受体蛋白产品优势

HEK293细胞表达：实现翻译后修饰及蛋白正确折叠

多种属：Human, Mouse, Cynomolgus/Rhesus macaque, Rat, Porcine, Rabbit, Feline, Bovine，可全面应用于不同种属交叉实验

高纯度：SDS-PAGE验证纯度＞95%，SEC-MALS验证纯度＞90%

低内毒素：<1.0 EU/μg

亲和力经SPR&BLI验证: 活性有保障，免费提供实验方案

高稳定性：严格质控为高批间一致性保驾护航

提供生物素化定点标记蛋白：采用Avitag™定点标记的生物素化Fc受体蛋白，标记效率高且标记位点专一明确，方便药物开发及工艺优化过程中基于与链霉亲和素结合的ELISA/SPR/BLI检测

验证数据

产品优势

高纯度

为了满足抗体药研发过程中对用于检测和筛选的蛋白质纯度高的需求，我们的QC团队对大多数重组蛋白都同时进行了SDS-PAGE和SEC-MALS分析。值得注意的是，Fc受体的单体形式反映了其天然状态，应该使用单体纯度尽可能高的Fc受体来验证和抗体的亲和力，以避免因聚集效应而产生的结合活性虚高现象。因此，我们通过SEC-MALS检测建立了严格的单体纯度内部控制标准，只有满足标准的批次才会被放行。

Fc受体蛋白-SPR
图1. 经SDS-PAGE验证，Human CD32a (R167), His Tag (Cat. No. CDA-H5221) 纯度高于 95%；经 SEC-MALS 验证，Human CD32a (R167), His Tag (Cat. No. CDA-H5221) 纯度高于 90%，分子量为25-35 kDa。
亲和力经SPR&BLI验证

分子间的相互作用可以通过多种方法检测。传统的ELISA方法不适合检测像CD16和CD32这样的低亲和力受体与抗体的相互作用，而SPR则成为研究Fc受体与抗体亲和力最常用的方法。为了保证我们的Fc受体能够达到预期的性能，我们的QC团队使用Biacore平台对我们大部分产品进行测试。此外，为了满足客户在抗体药研发过程中不同的应用场景，我们同样采用BLI技术进行了亲和力的验证。所有展现在网页上的SPR&BLI数据都免费提供内部实验操作方法供参考。

FCA-H52H1-S1
图2. SPR验证，固定在CM5芯片上的Human CD64, His Tag (Cat. No. FCA-H52H1) 可以结合Herceptin ( 赫赛汀单抗 )，亲和常数为4.92 nM ( Biacore 8K )。
申请SPR Protocol
点击咨询SPR服务
Fc受体蛋白-BLI
图3. SPR验证，将Human FCGRT&B2M Heterodimer Protein (Cat. No. FCM-H5286)通过anti-His抗体固定在CM5芯片上，可以结合Herceptin (赫赛汀单抗)，亲和常数为0.261 μM (Biacore T200)。
申请SPR Protocol
点击咨询SPR服务
Fc受体蛋白-BLI
图4. BLI验证，将Human CD64, His Tag (SPR & BLI verified) (Cat. No. FCA-H52H1)固定在HIS1K生物传感器上，可以结合Herceptin (赫赛汀单抗)，亲和常数为6.98 nM (ForteBio Octet Red96e)。
申请BLI Protocol
点击咨询BLI服务
Fc受体蛋白-BLI
图5. BLI验证，将Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7)通过生物素化的his antibody 固定在SA传感器上，可以结合Herceptin (赫赛汀单抗)，亲和常数为0.14 μM (ForteBio Octet Red96e)。
申请BLI Protocol
点击咨询BLI服务
高批间一致性

我们通过采取严格的质量控制措施，以确保产品的批间一致性。如下图所示，被测样品的批间差异很小。

Fc受体蛋白-高批间一致性
图6. SPR验证，固定在CM5芯片上的Human Fc gamma RIIB / CD32b Protein (Cat. No. CDB-H5228)可以结合Rituximab (利妥昔单抗)，亲和常数为10μM (Biacore T200)。
申请SPR Protocol
点击咨询SPR服务

用相似度评分软件分析可以看到，不同批次的CD32b相似度非常高，体现了高批间一致性。

Fc受体蛋白-高批间一致性
图7. 不同批次的Human Fc gamma RIIB / CD32b ( Cat. No. CDB-H5228 ) 相似度在90%以上。
产品列表
  • FcRn
  • FcγR
  • FcεR
  • FcαR
分子 货号 表达系统 产品描述 结构
FcRn (FCGRT & B2M)FRT-01-Human FcRn binding Kit (TR-FRET)New
CHEK-ATP079-HEK293/Human FcRn (FCGRT & B2M) Stable Cell LineHot
CHEK-ATP132-HEK293/FcRn (FCGRT & B2M), GFP Tag Stable Cell Line
FCM-H5283HEK293Human FcRn / FCGRT&B2M Heterodimer Protein, Strep Tag&Tag Free (MALS & SPR verified)Hot
FCM-C82W9HEK293Biotinylated Canine FcRn / FCGRT&B2M Heterodimer Protein, His,&Tag Free (MALS & SPR verified)Hot
FCM-H5286HEK293Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI & HPLC-verified)
FCM-H8286HEK293Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, ultra sensitivity (primary amine labeling) (HPLC & SPR verified)
FCM-H82W4HEK293Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, ,His Tag&Strep II Tag (SPR & BLI & HPLC verified)
FCM-H82W7HEK293Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, His, (MALS & SPR verified)
FCN-H52W7HEK293Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI & MALS verified)
FCM-M52W2HEK293Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (MALS & BLI verified)
FCM-M52W8HEK293Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (MALS & BLI verified)
FCM-M82W5HEK293Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, His, (MALS & BLI verified)
FCM-M82W6HEK293Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, ,His Tag&Twin-Strep Tag (MALS & BLI-verified)
FCM-C5284HEK293Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI & MALS verified)
FCM-C52W9HEK293Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His Tag (MALS & BLI verified)
FCM-C82W3HEK293Biotinylated Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His, (MALS & SPR verified)
FCM-C82W5HEK293Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,&Strep II Tag (HPLC&SPR&BLI verified)
FCM-R5287HEK293Rat FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (MALS & SPR verified)
FCM-R82W7HEK293Biotinylated Rat FcRn / FCGRT&B2M Heterodimer Protein, ,His Tag&Strep II Tag (MALS & SPR verified)
FCM-P5280HEK293Porcine FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (MALS & SPR verified)
FCM-R52W5HEK293Rabbit FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (MALS & SPR verified)
FCM-R82W6HEK293Biotinylated Rabbit FcRn / FCGRT&B2M Heterodimer Protein, His,&Tag Free (MALS&SPR verified)
FCN-F82W3HEK293Biotinylated Feline FcRn / FCGRT&B2M Heterodimer Protein, His, (MALS & SPR verified)
FCN-B82W3HEK293Biotinylated Bovine FcRn / FCGRT&B2M Heterodimer Protein, His, (MALS & SPR verified)
FCM-C52W8HEK293Canine FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (MALS & SPR verified)
分子 货号 表达系统 产品描述 结构
Fc gamma RI / CD64FCA-H52H1HEK293Human Fc gamma RI / CD64 Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RI / CD64FCA-H82E8HEK293Biotinylated Human Fc gamma RI / CD64 Protein, His, (SPR & BLI & MALS verified)
Fc gamma RI / CD64CD4-M5227HEK293Mouse Fc gamma RI / CD64 Protein, His Tag (MALS & BLI verified)
Fc gamma RI / CD64CD4-M82E7HEK293Biotinylated Mouse Fc gamma RI / CD64 Protein, His, (MALS & SPR verified)
Fc gamma RI / CD64FCA-C52H6HEK293Cynomolgus Fc gamma RI / CD64 Protein, His Tag (MALS & SPR verified)
Fc gamma RI / CD64FCA-C82E8HEK293Biotinylated Cynomolgus Fc gamma RI / CD64 Protein, His, (MALS & BLI verified)
Fc gamma RI / CD64CD4-R52H3HEK293Rat Fc gamma RI / CD64 Protein, His Tag (MALS verified)
Fc gamma RI / CD64FCA-C52H8HEK293Canine Fc gamma RI / CD64 Protein, His Tag (MALS & SPR verified)
Fc gamma RIIA / CD32aCD1-H5223HEK293Human Fc gamma RIIA / CD32a (H167) Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIA / CD32aCDA-H5221HEK293Human Fc gamma RIIA / CD32a (R167) Protein, His Tag (MALS & BLI verified)
Fc gamma RIIA / CD32aCDA-H5283HEK293Human Fc gamma RIIA / CD32a (R167) Protein, Strep Tag (MALS & SPR verified)
Fc gamma RIIA / CD32aCDA-H5284HEK293Human Fc gamma RIIA / CD32a (H167) Protein, Strep Tag (MALS & SPR verified)
Fc gamma RIIA / CD32aCDA-H82E5HEK293Biotinylated Human Fc gamma RIIA / CD32a (R167) Protein, His, (MALS & SPR verified)
Fc gamma RIIA / CD32aCDA-H82E6HEK293Biotinylated Human Fc gamma RIIA / CD32a (H167) Protein, ,His Tag (SPR & BLI & MALS verified)
Fc gamma RIIA / CD32aCDA-C52H7HEK293Cynomolgus Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIA / CD32aCDA-C82E5HEK293Biotinylated Cynomolgus Fc gamma RIIA / CD32a Protein, His, (SPR & BLI & MALS verified)
Fc gamma RIIA / CD32aCDA-R52H5HEK293Rat Fc gamma RIIA / CD32a Protein, His Tag (MALS & SPR verified)
Fc gamma RIIA / CD32aCDA-R52H4HEK293Rhesus macaque Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIB / CD32bCDB-M52H7HEK293Mouse Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIB / CD32bCDB-M82E8HEK293Biotinylated Mouse Fc gamma RIIB / CD32b Protein, ,His Tag (SPR & BLI & MALS verified)
Fc gamma RIIB / CD32bCDB-C5224HEK293Cynomolgus Fc gamma RIIB / CD32b Protein, His Tag (MALS & SPR verified)
Fc gamma RIIB / CD32bCDB-C82E4HEK293Biotinylated Cynomolgus Fc gamma RIIB / CD32b Protein, His, (SPR & BLI & MALS verified)
Fc gamma RIIB / CD32bCDB-R52H3HEK293Rat Fc gamma RIIB / CD32b Protein, His Tag (MALS verified)
Fc gamma RIIB/C / CD32b/cCDB-H5228HEK293Human Fc gamma RIIB/C (CD32b/c) Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIB/C / CD32b/cCDB-H5284HEK293Human Fc gamma RIIB/C (CD32b/c) Protein, Strep Tag (MALS & SPR verified)
Fc gamma RIIB/C / CD32b/cCDB-H5298HEK293Human Fc gamma RIIB/C (CD32b/c) Protein, SUMO,His Tag (MALS & BLI verified)
Fc gamma RIIB/C / CD32b/cCDB-H52S9HEK293Human Fc gamma RIIB/C (CD32b/c) Protein, HSA,His Tag (MALS & BLI verified)
Fc gamma RIIB/C / CD32b/cCDB-H82E0HEK293Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein, ,His Tag (SPR & BLI & MALS verified)
Fc gamma RIII / CD16CDA-M52H8HEK293Mouse Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIII / CD16FC6-M82E0HEK293Biotinylated Mouse Fc gamma RIII / CD16 Protein, His, (MALS & SPR verified)
Fc gamma RIII / CD16FC6-C52H9HEK293Cynomolgus Fc gamma RIII / CD16 Protein, His Tag (MALS & BLI verified)
Fc gamma RIII / CD16FC6-C82E0HEK293Biotinylated Cynomolgus Fc gamma RIII / CD16 Protein, His, (MALS & BLI verified)
Fc gamma RIII / CD16FC6-R52H6HEK293Rhesus macaque Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIIA / CD16aCD8-H52H4HEK293Human Fc gamma RIIIA / CD16a (V176) Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIIA / CD16aCDA-H5220HEK293Human Fc gamma RIIIA / CD16a (F176) Protein, His Tag (MALS & SPR verified)
Fc gamma RIIIA / CD16aCDA-H5290HEK293Human Fc gamma RIIIA / CD16a (V176) Protein, SUMO,His Tag (MALS & BLI verified)
Fc gamma RIIIA / CD16aCDA-H52H6HEK293Human Fc gamma RIIIA / CD16a (F176, S197P) Protein, His Tag (MALS&SPR verified)
Fc gamma RIIIA / CD16aCDA-H52S1HEK293Human Fc gamma RIIIA / CD16a (V176) Protein, HSA,His Tag (MALS & BLI verified)
Fc gamma RIIIA / CD16aCDA-H82E8HEK293Biotinylated Human Fc gamma RIIIA / CD16a (F176) Protein, ,His Tag (SPR & BLI & MALS verified)
Fc gamma RIIIA / CD16aCDA-H82E9HEK293Biotinylated Human Fc gamma RIIIA / CD16a (V176) Protein, ,His Tag (HPLC & SPR & BLI verified)
Fc gamma RIIIB / CD16b (NA1)CDB-H5227HEK293Human Fc gamma RIIIB / CD16b (NA1) Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIIB / CD16b (NA1)CDB-H5296HEK293Human Fc gamma RIIIB / CD16b (NA1) Protein, SUMO,His Tag (MALS & BLI-verified)
Fc gamma RIIIB / CD16b (NA1)CDB-H82E4HEK293Biotinylated Human Fc gamma RIIIB / CD16b (NA1) Protein, His, (SPR & BLI & MALS verified)
Fc gamma RIIIB / CD16b (NA2)CDB-H5222HEK293Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI & MALS verified)
Fc gamma RIIIB / CD16b (NA2)CDB-H5283HEK293Human Fc gamma RIIIB / CD16b (NA2) Protein, Strep Tag (MALS & SPR verified)
Fc gamma RIIIB / CD16b (NA2)CDB-H5294HEK293Human Fc gamma RIIIB / CD16b (NA2) Protein, SUMO,His Tag (MALS & BLI-verified)
Fc gamma RIIIB / CD16b (NA2)CDB-H82EaHEK293Biotinylated Human Fc gamma RIIIB / CD16b (NA2) Protein, His, (SPR & BLI & MALS verified)
Fc gamma RIV / CD16-2FC4-M52H3HEK293Mouse Fc gamma RIV / CD16-2 Protein, His Tag (MALS verified)
Fc gamma RIV / CD16-2FC4-M82E8HEK293Biotinylated Mouse Fc gamma RIV / CD16-2 Protein, His, (HPLC & BLI verified)
分子 货号 表达系统 产品描述 结构
CD23CD3-H5249HEK293Human CD23 / Fc epsilon RII Protein, His Tag (MALS verified)
CD23CD3-H82Q5HEK293Biotinylated Human CD23 / Fc epsilon RII Protein, His, (MALS & SPR verified)
Fc epsilon RI alphaFCA-H5228HEK293Human Fc epsilon RI alpha Protein, His Tag (MALS verified)
Fc epsilon RI alphaFCA-H5259HEK293Human Fc epsilon RI alpha Protein, Fc Tag (MALS & BLI verified)
Fc epsilon RI alphaFCA-H82E3HEK293Biotinylated Human Fc epsilon RI alpha Protein, His, (MALS & BLI verified)
Fc epsilon RI alphaFCA-C5255HEK293Cynomolgus Fc epsilon RI alpha Protein, Fc Tag (BLI verified)
Fc epsilon RI alphaFCA-C52H9HEK293Cynomolgus Fc epsilon RI alpha Protein, His Tag (SPR verified)
分子 货号 表达系统 产品描述 结构
CD89CD9-H52HaHEK293Human CD89 / FCAR Protein, His Tag (MALS verified)

Fc受体相关分子-IgG Fc protein

IgG Fc IgG1 Fc IgG2 Fc IgG2a Fc IgG2b Fc IgG4 Fc IgG3 Fc
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