膜杰作
Star Staining
分子别名（Synonym）
CD48,BCM1,SLAMF2,BLAST,BLAST1,MEM-102,TCT.1,BCM-1,SLAMF-2,BLAST-1
表达区间及表达系统（Source）
Human CD48, Fc Tag (BC1-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ser 220 (Accession # P09326-1).
Predicted N-terminus: Gln 27
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 49.0 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human CD48, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human 2B4, His Tag (Cat. No. 2B4-H5224) at 2 μg/mL (100 μL/well) can bind Human CD48, Fc Tag (Cat. No. BC1-H5255) with a linear range of 0.08-10 μg/mL (QC tested).
Protocol
Immobilized Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (Cat. No. 2B4-H82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human CD48, Fc Tag (Cat. No. BC1-H5255) with a linear range of 0.3-5 ng/mL (Routinely tested).
Protocol
背景（Background）
CD48 antigen is also known as Signaling lymphocytic activation molecule 2, SLAM family member 2 (SLAMF2), Leukocyte antigen MEM-102, BCM1 surface antigen, B-lymphocyte activation marker BLAST-1. CD48 is a 65 kDa GPI-linked protein which belongs to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 antigen contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domain. CD48 interacts with CD244 in an heterophilic manner. CD48 is ligand for CD2. CD48 might facilitate interaction between activated lymphocytes. CD48 is probably involved in regulating T-cell activation.
