膜杰作
Star Staining
分子别名（Synonym）
CD3E & CD3D,CD3 delta & CD3 epsilon
表达区间及表达系统（Source）
Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (CDD-H5258) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
Request for sequence
蛋白结构（Molecular Characterization）
Human CD3E & CD3D Heterodimer Protein, Llama IgG2b Fc Tag & Llama IgG2b Fc Tag, low endotoxin is produced by co-expression of CD3E and CD3D, has a calculated MW of 42.9 kDa (CD3E) and 40.8 kDa (CD3D). Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 50-60 kDa due to glycosylation.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (Cat. No. CDD-H5258) at 2 μg/mL (100 μL/well) can bind SP34-2 (mouse IgG1) with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
