膜杰作
Star Staining
分子别名（Synonym）
IL13,ALRH,BHR1,MGC116786,MGC116788,MGC116789,P600,Interleukin-13
表达区间及表达系统（Source）
Human IL-13 Protein, His Tag (IL3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1).
Predicted N-terminus: Gly 21
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 14.2 kDa. The protein migrates as 15 kDa and 25-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL-13 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human IL-13 Protein, His Tag (Cat. No. IL3-H52H4) at 5 μg/mL (100 μL/well) can bind Human IL-13RA2, Fc Tag (Cat. No. IL2-H5256) with a linear range of 5-39 ng/mL (QC tested).
Protocol
Immobilized Human IL-13 Protein, His Tag (Cat. No. IL3-H52H4) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL-13 Antibody, Human IgG4 with a linear range of 0.3-5 ng/mL (Routinely tested).
Protocol
Immobilized Mouse IL-13RA1, Fc Tag (Cat. No. IL1-M5258) at 2 μg/mL (100 μL/well) can bind Human IL-13 Protein, His Tag (Cat. No. IL3-H52H4) with a linear range of 5-39 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human IL-13 R alpha 1 Protein, His,Avitag (Cat. No. IL1-H82E8) captured on Biotin CAP-Series S Sensor Chip can bind Human IL-13 Protein, His Tag (Cat. No. IL3-H52H4) with an affinity constant of 34.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Mouse IL-13RA1, Fc Tag (Cat. No. IL1-M5258) on Protein A Biosensor, can bind Human IL-13 Protein, His Tag (Cat. No. IL3-H52H4) with an affinity constant of 22.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Monoclonal Anti-Human IL-13 Antibody, Human IgG4 on Protein A Biosensor, can bind Human IL-13 Protein, His Tag (Cat. No. IL3-H52H4) with an affinity constant of 4.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）-Bioactivity CELL BASE
Human IL-13 Protein, His Tag (Cat. No. IL3-H52H4) stimulates proliferation of TF-1 cells. The EC50 for this effect is 0.05-0.08 μg/mL (Routinely tested).
Protocol
背景（Background）
Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.
