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 >  Protein>Spike S1 >S1N-C52H3

SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified)

DS MSDS COA
我们也有适用于血清学检测的该产品形式，敬请了解。

分子别名（Synonym）

Spike,S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1

表达区间及表达系统（Source）

SARS-CoV-2 S1 protein, His Tag (S1N-C52H3) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1).

Predicted N-terminus: Val 16

Request for sequence

蛋白结构（Molecular Characterization）

Spike S1 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳（SDS-PAGE）

Spike S1 SDS-PAGE

SARS-CoV-2 S1 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Spike S1 SEC-MALS

The purity of SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H3) is more than 85% and the molecular weight of this protein is around 101-124 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Spike S1 ELISA

Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H3) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

 

背景（Background）

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

文献引用（Citations）

 

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