分子别名(Synonym)
Envelope protein,Env polyprotein,Envelope glycoprotein,env
表达区间及表达系统(Source)
SARS-CoV-2 Envelope protein, GST,His Tag (ENN-C5128) is expressed from E. coli cells. It contains AA Met 1 - Val 75 (Accession # QHU79206.1).
Predicted N-terminus: Met
蛋白结构(Molecular Characterization)
This protein carries a GST tag at the N-terminus and a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.8 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE).
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, Arginine, pH8.0 with sucrose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
SARS-CoV-2 Envelope protein, GST,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
背景(Background)
The SARS-CoV-2 is composed of a double-layered lipid envelope, including Spike glycoprotein (S), Envelope protein (E), Membrane glycoprotein (M), and Nucleocapsid protein (Nucleocapsid protein, N). Among them, The amino acid sequence of the SARS-CoV-2 envelope protein is 95% identical of the SARS envelope protein.