膜杰作
Star Staining
分子别名（Synonym）
IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D
表达区间及表达系统（Source）
Human IFN-alpha 1, His Tag (IFA-H52H9) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1).
Predicted N-terminus: Cys 24
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.3 kDa. The protein migrates as 22-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IFN-alpha 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-BLI
Loaded Human IFN-alpha 1, His Tag (Cat. No. IFA-H52H9) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. IF1-H5253) with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IFN-alpha 1, His Tag (Cat. No. IFA-H52H9) on HIS1K Biosensor, can bind Human IFNAR2, Fc Tag (Cat. No. IF2-H5255) with an affinity constant of 19.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Interferon alpha-1 is also known as IFN-alpha-1/13, Interferon alpha-D, LeIF D and IFNA1, belongs to the alpha / beta interferon family. Interferons alpha-1 and alpha-13 have identical protein sequences. Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha can either suppress or promote the development of autoimmune diabetes. It is likely that IFN-alpha plays a complex role in the etiology of type 1 diabetes.
