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Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47) (Trehalose free)

抗体来源(Source)

Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47) (NUN-S47) was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARS-CoV-2 Nucleocapsid protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. ELISA test validated that this antibody can bind multiple N protein variants (Cat. No. NUN-C52H8, NUN-C52Hc, NUN-C52Hd) with similar affinity as compared to the wild type N protein (Cat. No. NUN-C5227).

克隆号(Clone)

AS47

种属(Species)

Mouse

亚型(Isotype)

Mouse IgG1 | Mouse Kappa

偶联(Conjugate)

Unconjugated

种属反应性(Reactivity)

Virus

特异性(Specificity)

This product is a specific antibody against SARS-CoV-2 Nucleocapsid protein. No cross-reactivity is detected with nucleocapsid protein of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.

应用(Application)

ApplicationRecommended Usage
ELISA0.1-10 ng/mL

纯度(Purity)

>95% as determined by SDS-PAGE.

纯化(Purification)

Protein A purified/ Protein G purified

制剂(Formulation)

Supplied as 0.2 μm filtered solution in PBS, pH7.4 .

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with blue ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. For long term storage, the product is stable for up to 3 years at -70°C from date of receipt;
  2. For short term storage, the product is stable for up to 12 months at 2-8°C from date of receipt.
 

电泳(SDS-PAGE)

Nucleocapsid protein SDS-PAGE

Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Nucleocapsid protein ELISA

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47) (Cat. No. NUN-S47) with a linear range of 0.15-2.5 ng/mL (QC tested).

Protocol

 

背景(Background)

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

文献引用(Citations)

 

前沿进展

 
 
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Nucleocapsid protein靶点信息
英文全称:Nucleocapsid proteins
中文全称:核壳蛋白
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
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