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Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Trehalose free) (MALS verified)

抗体来源(Source)

Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (S1N-M13A1) is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified recombinant SARS-CoV-2 Spike S1 Protein.

克隆号(Clone)

AM130

亚型(Isotype)

Human IgG1 | Human Kappa

偶联(Conjugate)

Unconjugated

种属反应性(Reactivity)

Virus

特异性(Specificity)

This product can recognize SARS-CoV-2 and SARS-CoV Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.

应用(Application)

ApplicationRecommended Usage
ELISA0.1-20 ng/mL

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

纯化(Purification)

Protein A purified/ Protein G purified

制剂(Formulation)

Supplied as 0.2 μm filtered solution in PBS, pH7.4 .

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. For long term storage, the product is stable for up to 3 years at -70°C from date of receipt;
  2. For short term storage, the product is stable for up to 12 months at 2-8°C from date of receipt.
 

电泳(SDS-PAGE)

Spike RBD SDS-PAGE

Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Spike RBD SEC-MALS

The purity of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Cat. No. S1N-M13A1) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Spike RBD ELISA

Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H2) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Cat. No. S1N-M13A1) with a linear range of 0.078-1.25 ng/mL (QC tested).

Protocol

 

背景(Background)

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

前沿进展

 
 
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