膜杰作
Star Staining
抗体来源（Source）
Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS58) (S1N-S58), originally from mouse immunized with recombinant SARS-CoV-2 Spike S1 protein, is produced from hybridoma.
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Hybridoma Monoclonal
种属反应性（Reactivity）
Virus
特异性（Specificity）
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.15-20 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4 .
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- For short term storage, the product is stable for up to 12 months at 2-8°C from date of receipt.
电泳（SDS-PAGE）
Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H1) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (Cat. No. S1N-S58) with a linear range of 0.15-2.5 ng/mL (QC tested).
Protocol
Detection SARS-CoV-2 S protein RBD by Sandwich ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (Cat. No. S1N-S58) at 1 μg/mL (100 μL/well) can bind S protein RBD. And then add Anti-SARS-CoV-2 Spike S1 Antibody at 1:5000. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 5 pg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (Cat. No. S1N-S58) captured on CM5 chip via Anti-mouse antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 0.246 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
背景（Background）
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
