膜杰作
Star Staining
分子别名（Synonym）
Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
表达区间及表达系统（Source）
SARS-CoV-2 S protein RBD (F490L), His Tag (SPD-C52Hf) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (F490L)).
Predicted N-terminus: Arg 319
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
SARS-CoV-2 S protein RBD (F490L), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (Cat. No. SPD-M128) were added into SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) and SARS-CoV-2 (COVID-19) S protein RBD, His Tag (Cat.No. SPD-C52H3) : Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified) (Cat.No. AC2-H82E6) binding reactions. The half maximal inhibitory concentrations (IC50) of SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) and SARS-CoV-2 (COVID-19) S protein RBD, His Tag (Cat.No. SPD-C52H3) are 0.20444 μg/mL and 0.78328 μg/mL respectively (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) with an affinity constant of 53.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) with an affinity constant of 57.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) on AHC Biosensor, can bind SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) with an affinity constant of 45.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
