膜杰作
Star Staining
分子别名（Synonym）
TOP2A,TOP2,DNA topoisomerase 2-alpha,DNA topoisomerase II,alpha isozyme
表达区间及表达系统（Source）
Human TOP2A, Tag Free (TOA-H5117) is expressed from E. coli cells. It contains AA Ser 1354 - Ser 1473 (Accession # P11388-1).
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 12.8 kDa. The protein migrates as 20 kDa under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 0.5 M KCl, pH8.0 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied as sterile liquid solution and shipped frozen with dry ice.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Human TOP2A, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
背景（Background）
DNA topoisomerase 2-alpha (TOP2A) is also known as DNA topoisomerase II, alpha isozyme and TOP2. It is a topoisomerase which control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. Also it is the targets of anti-cancer drugs that cause treatment-related leukemias with balanced translocations.
