膜杰作
Star Staining
抗体来源（Source）
PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (FM3-HPY53) is produced via site-specific conjugation of PE to Monoclonal Anti-FMC63 Antibody, Mouse IgG1 under optimal conditions with a proprietary technology. We also carry another proclin-containing version of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (FM3-PY54A2) produced with the same production process except for proclin, and the proclin-containing product has the same performance and can be stored under 2-8 °C for no less than 12 months after reconstitution.
Besides, we carry another premium grade PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (FM3-PY54G0), produced with the same production process but under more rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. It is designed for cell isolation and cell culture applications in the early preclinical stage.
应用（Application）
Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression). Please note that this product is NOT compatible to streptavidin detection system.
克隆号（Clone）
Y45
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
特异性（Specificity）
Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
免疫原（Immunogen）
Recombinant FMC63 scFv derived from HEK293 cells.
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
同型对照（Isotype Control）
The Isotype control is sold separately and you can search for Cat. No. DNP-PM1 for product information.
推荐稀释比（Recommended Dilution）
1:50
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
活性（Bioactivity）-FACS
5e5 of the Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-HPY53) and PE-Labeled Monoclonal Mouse IgG1 Antibody Isotype Control (Cat. No. DNP-PM1) respectively, PE signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-HPY53) binding to CD3+ cells present in human PBMC. Human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
背景（Background）
FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
