膜杰作
Star Staining
分子别名（Synonym）
LGR5, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Leucine-rich repeat-containing G-protein coupled receptor 5
表达区间及表达系统（Source）
Human LGR5, His Tag (LG5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Arg 561 (Accession # O75473-1).
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 62.0 kDa. The protein migrates as 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human LGR5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human R-Spondin 3 Protein, Fc Tag (Cat. No. RS3-H5253) at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag (Cat. No. LG5-H52H3) with a linear range of 10-78 ng/mL (QC tested).
Protocol
Immobilized Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag (Cat. No. LG5-H52H3) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human R-Spondin 3 (22-146), His Tag (Cat. No. RS6-H5222) with immobilized on CM5 Chip can bind Human LGR5, His Tag (Cat. No. LG5-H52H3) with an affinity constant of 18.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
