膜杰作
Star Staining
分子别名（Synonym）
IL34,C16orf77,IL-34,Interleukin-34,MGC34647
表达区间及表达系统（Source）
Human IL-34, His Tag (IL4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 21 - Pro 242 (Accession # Q6ZMJ4-1).
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.3 kDa. The protein migrates as 32 kDa and 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL-34, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human M-CSF R, Mouse IgG2a Fc Tag (MALS verified) (Cat. No. CSR-H5255) at 5 μg/mL (100 μL/well) can bind Human IL-34, His Tag (Cat. No. IL4-H52H3) with a linear range of 0.4-2 ng/mL (QC tested).
Protocol
Immobilized Human TREM2, Fc Tag (Cat. No. TR2-H5254) at 5 μg/mL (100 μL/well) can bind Human IL-34, His Tag (Cat. No. IL4-H52H3) with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
背景（Background）
nterleukins (IL) are a group of cytokines that play an important role in the immune system. They modulate inflammation and immunity by regulating growth, mobility and differentiation of lymphoid and other cells.This entry represents interleukin-34 (IL-34), it was identified via functional screening of a library of secreted proteins [1]. This cytokine promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R)
