膜杰作
Star Staining
| ID | Components | Size |
| RAS027-C01 | Pre-coated SARS-CoV-2 Spike Trimer(B.1.1.7) Microplate | 1 plate |
| RAS027-C02 | Positive Control | 100 μL |
| RAS027-C03 | Negative Control | 100 μL |
| RAS027-C04 | HRP-Anti-Human IgG | 200 μL |
| RAS027-C05 | 10xWashing Buffer | 50 mL |
| RAS027-C06 | Dilution Buffer | 50 mL |
| RAS027-C07 | Substrate Solution | 12 mL |
| RAS027-C08 | Stop Solution | 7 mL |
背景（Background）
Multiple variants of SARS-CoV-2 are circulating globally and posting new challenges to human health. The variant B.1.1.7 identified in the United Kingdom (UK) carries a large number of mutations and is reported to spread more easily and quickly than other variants. There are 9 mutations on the spike protein of the U.K. variant: HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H. To evaluate the impacts of the new variants, a rapid and effective assay kit detecting the levels of antibody against the mutant is in urgent need.
应用说明（Application）
This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 Spike antibody in serum/plasma in vitro. The Spike antigen used in this kit contains 9 mutations (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) found in the U.K. variant (known as B.1.1.7, 20B/501Y.V1 or VOC 202012/01).
It is for research use only.
存储 & 运输（Storage & Shipping）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike Protein. The kit consists of Pre-coated SARS-CoV-2 Spike Protein Microplate, an Positive Control, an Negative Control, an HRP-Anti-Human IgG secondary antibody and related buffer.
Your experiment will include 4 simple steps:
a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
典型数据-Typical Data Please refer to Ds document for the assay protocol.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Trimer at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG in 1:800 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 78 ng/mL (QC tested).
