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 >  Protein>uPAR >UPR-HF2H3

FITC-Labeled Human uPAR / PLAUR Protein, His Tag

分子别名(Synonym)

uPAR,PLAUR,CD87,MO3

表达区间及表达系统(Source)

FITC-Labeled Human uPAR, His Tag (UPR-HF2H3) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Arg 303 (Accession # Q03405-1).

Predicted N-terminus: Leu 23

Request for sequence

蛋白结构(Molecular Characterization)

uPAR Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 32.3 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 1-3.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

uPAR SDS-PAGE

FITC-Labeled Human uPAR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

uPAR ELISA

Immobilized Anti-uPAR Antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human uPAR, His Tag (Cat. No. UPR-HF2H3) with a linear range of 10-156 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-FACS

uPAR FACS

2e5 of Anti-uPAR CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human uPAR, His Tag (Cat. No. UPR-HF2H3) and negative control protein respectively, washed and analyzed with FACS (QC tested).

Protocol

 

背景(Background)

Urokinase plasminogen activator surface receptor (U-PAR) is also known as PLAUR, Monocyte activation antigen Mo3, CD antigen CD87. PLAUR contains three UPAR/Ly6 domains. U-PAR is expressed in neurons of the rolandic area of the brain (at protein level) and is also expressed in the brain. PLAUR / CD87 interacts with MRC2, SRPX2 and SORL1. PLAUR / UPAR acts as a receptor for urokinase plasminogen activator and plays a role in localizing and promoting plasmin formation. U-PAR mediates the proteolysis-independent signal transduction activation effects of U-PA.

 

前沿进展

 
 
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uPAR靶点信息
英文全称:Urokinase plasminogen activator surface receptor
中文全称:尿激酶型纤溶酶原激活物表面受体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:5详情
最高研发阶段:临床二期
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