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Human EGF Protein, His Tag

分子别名(Synonym)

EGF,URG,HOMG4

表达区间及表达系统(Source)

Human EGF Protein, His Tag (EGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).

Request for sequence

蛋白结构(Molecular Characterization)

EGF Structure

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

无菌(Sterility)

Negative

支原体(Mycoplasma)

Negative.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

EGF SDS-PAGE

Human EGF Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-Organoid Culture

EGF ORGANOID CULTURE

EGF (Cat. No. EGF-H52H3) maintains intestinal organoid growth well through multiple passages and long-term culture, comparable to competing companies. The organoids showed good budding morphology.

EGF ORGANOID CULTURE

Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 (Cat. No. RS6-H4220), FGF7 (Cat. No. FG7-H52H5), FGF10, HGF (Cat. No. HGF-H52H3) actively support liver ductal organoid growth.

 

活性(Bioactivity)-ELISA

EGF ELISA

Immobilized Human EGF Protein, His Tag (Cat. No. EGF-H52H3) at 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.2-5 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-Bioactivity CELL BASE

EGF CELL

Human EGF Protein, His Tag (Cat. No. EGF-H52H3) stimulates proliferation of EGFR (Luc) HEK293 Reporter Cell. The specific activity of Human EGF Protein, His Tag is > 3.00 X 10^5 IU/mg, which is calibrated against human growth factor EGF WHO International Standard (NIBSC code: 91/530) (QC tested).

Protocol

 

背景(Background)

Human epidermal growth factor (EGF) is also known as HOMG4 and  URG,and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of D synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk.

 

前沿进展

 
 
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EGF靶点信息
英文全称:Epidermal growth factor
中文全称:表皮生长因子
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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