膜杰作
Star Staining
抗体来源（Source）
Anti-Trastuzumab Antibody (AY1b) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
AY1b
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1/kappa
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Trastuzumab.
特异性（Specificity）
Recognizes Trastuzumab specifically, no cross reactivity with other humanized antibodies.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.39-100 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Anti-Trastuzumab Antibody (AY1b) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Trastuzumab at 1 μg/mL (100 μL/well) can bind Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) at 2 μg/mL, add increasing concentrations of Trastuzumab in the 10% human serum and then add Biotinylated Anti-Trastuzumab Antibodies at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 48 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) was detected by Biotinylated Human Her2, His,Avitag (Cat. No. HE2-H82E2) with a half maximal inhibitory concentration (IC50) of 4.58 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Trastuzumab captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Trastuzumab Antibody (AY1b) (Cat. No. TRB-Y1b) with an affinity constant of 0.729 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
Trastuzumab, is a monoclonal antibody used to treat breast cancer. Specifically it is used for breast cancer that is HER2 receptor positive. Trastuzumab works by binding to the HER2 receptor and slowing down cell duplication. Trastuzumab is a recombinant IgG1 kappa, humanized monoclonal antibody that selectively binds with high affinity in a cell-based assay (Kd = 5 nM) to the extracellular domain of the human epidermal growth factor receptor protein.
