登录 | 注册    关注公众号  
微信公众号
搜索
 >  Antibody>Adalimumab >ADB-Y23b

Anti-Adalimumab Antibody (AY23b) (recommended for PK/PD)

抗体来源(Source)

Anti-Adalimumab Antibody (AY23b) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

克隆号(Clone)

AY23b

种属(Species)

Mouse

亚型(Isotype)

Mouse IgG1/kappa

抗体类型(Antibody Type)

Recombinant Monoclonal

种属反应性(Reactivity)

Human

免疫原(Immunogen)

Adalimumab.

特异性(Specificity)

Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.

应用(Application)

ApplicationRecommended Usage
ELISA0.2-100 ng/mL

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

纯化(Purification)

Protein A purified/ Protein G purified

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Adalimumab SDS-PAGE

Anti-Adalimumab Antibody (AY23b) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Adalimumab SEC-MALS

The purity of Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Adalimumab ELISA

Immobilized Adalimumab at 1 μg/mL (100 μL/well) can bind Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) with a linear range of 0.2-3 ng/mL (QC tested).

Protocol

Adalimumab ELISA

Immobilized Anti-Adalimumab Antibody (AY23b) (Cat. No. ADB-Y23b) at 2 μg/mL, add increasing concentrations of Adaliumumab and then add Biotinylated Biotinylated Anti-Adalimumab Antibody (AY17) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (QC tested).

Protocol

 

背景(Background)

Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant D technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定