膜杰作
Star Staining
| ID | Components | Size |
| RAS045-C01 | Pre-coated with SARS-CoV-2 Spike S1 Microplate | 1 plate |
| RAS045-C02 | Anti-SARS-CoV-2 Antibody (Control, Mouse IgG) | 100 μL |
| RAS045-C03 | SARS-CoV-2 Antibody Positive Control | 100 μL |
| RAS045-C04 | SARS-CoV-2 Antibody Negative Control | 100 μL |
| RAS045-C05 | HRP-Conjugated Antibody | 10 μg |
| RAS045-C06 | 10×Washing Buffer | 50 mL |
| RAS045-C07 | Dilution Buffer | 50 mL |
| RAS045-C08 | Substrate Solution | 12 mL |
| RAS045-C09 | Stop Solution | 7 mL |
背景（Background）
Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide. The SARS-CoV-2 pandemic presents an extraordinary challenge to the lives and health of the global population, resulting in a total of over 200 million diagnosed cases and 4.9 million death (as of October 2021). It also significantly affected the social, economic and environmental domains throughout the world and changed everyone’s life.
ACROBiosystems, a leading life science company dedicated to aid the global COVID-19 response，developed different kits suitable for serum antibody detection in different species (Mouse, Monkeys, humans) to meet the needs of preclinical and clinical experiments.
应用说明（Application）
This kit is developed for qualitative, titer or semi-quantitative measurement of Anti-SARS-CoV-2 Antibody IgG (Spike S1) in Mouse serum.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储 & 运输（Storage & Shipping）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Spike S1. The Kit consists of Pre-coated with SARS-CoV-2 Spike S1 Microplate, an Anti-SARS-CoV-2 Antibody (Control, Mouse IgG), an HRP-Anti-Goat anti mouse IgG secondary antibody.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.
b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calc μLated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
典型数据-Typical Data Please refer to Ds document for the assay protocol.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike S1 at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG in 1:100 mouse serum. Detection was performed using HRP-Anti-mouse IgG antibody with sensitivity of 9.8 ng/mL (QC tested).
