膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | IL-1 beta |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | ＜4.688pg/mL |
| Standard Curve Range | 4.688 pg/mL-150 pg/mL |
| Assay Time | 2 hr 50 min |
| Suitable Sample Type | For the detection and quantitative determination of IL-1 beta in human serum and cell culture supernatant. |
| Sample volume | 100 uL |
背景（Background）
IL-1 beta, a polypeptide immune mediator, is a potent proinflammatory cytokine that initiates and amplifies a wide variety of effects associated with innate immunity and host responses to microbial invasion and tissue injury. There are two types of IL-1 beta receptors, IL-1RI and IL-1RII, with the former transducing signals in response to ligand binding and the latter acting as a “decoy” receptor, binding ligand without transducing a signal.
应用说明（Application）
Human Interleukin-1 beta (IL-1 beta) ELISA Kit (Residue Testing) is developed for the detection and quantitative determination of GMP human IL-1 beta in samples from CAR-T product preparation processing.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
| ID | Components | Size |
| CRS012-C01 | Pre-coated Anti-IL-1 beta Antibody Microplate | 1 plate |
| CRS012-C02 | Human IL-1 beta Standard | 20 μg |
| CRS012-C03 | Biotin-Anti-IL-1 beta Antibody | 20 μg |
| CRS012-C04 | Streptavidin-HRP | 50 μL |
| CRS012-C05 | 10×Washing Buffer | 50 mL |
| CRS012-C06 | 2×Dilution Buffer | 50 mL |
| CRS012-C07 | Substrate Solution | 12 mL |
| CRS012-C08 | Stop Solution | 7 mL |
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human IL-1 beta. The kit consists of Pre-coated Anti-IL-1 beta Antibody Microplate and Human IL-1 beta Standard and Biotin-Anti-IL-1 beta Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Human IL-1 beta as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-IL-1 beta Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
