膜杰作
Star Staining
分子别名（Synonym）
EBI3,IL35,IL-35EBI3,IL35p35,Interleukin-35,IL-35
表达区间及表达系统（Source）
Human IL-35, Fc Tag (IL5-H5259) is expressed from human 293 cells (HEK293). It contains AA Arg 21 - Lys 229 (IL-27 beta) & Arg 23 - Ser 219 (IL-12 alpha) (Accession # Q14213-1 (IL-27 beta) & P29459-1 (IL-12 alpha)).
Predicted N-terminus: Arg 21
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 73.3 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL-35, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human IL-35 R, His Tag (Cat. No. ILR-H52H8) at 5 μg/mL (100 μL/well) can bind Human IL-35, Fc Tag (Cat. No. IL5-H5259) with a linear range of 0.002-0.625 μg/mL (QC tested).
Protocol
背景（Background）
Interleukin 35 (IL-35) is a recently discovered anti-inflammatory cytokine from the IL-12 family. Member of IL-12 family - IL-35 is produced by wide range of regulatory lymphocytes and plays a role in immune suppression. IL-35 can block the development of Th1 and Th17 cells by limiting early T cell proliferation. The heterodimeric cytokine of IL-35 involves two subunits of p35 and Ebi3 which are also seen in IL-12 and IL-27. IL-35 is recognized as a defi nite immunosuppressor with a huge potent of suppression.
