膜杰作
Star Staining
产品描述（Product Details）
Laminin-221 (LM221) takes multiple roles as a scaffold to store cells, growth factors and cytokines and is the most likely expressed cardiac laminin that maintains physiological activities of myocytes. Laminin 221-E8 consists of the C-terminal end of the alpha 2, beta 2, and gamma 1 chains. Previous study has confirmed that the laminin-221 heterotrimer (α2β2γ1) could bind integrin α7X1 and α7X2. Latest research indicated that the administration of LM221 enhanced accumulation of smooth muscle actin (SMA) and isolectin B4 (ILB4) double-positive cells to the damaged area and contributed to the improvement of cardiac function.
产品参数（Key parameter）
| Purity (SDS PAGE) | > 90% |
| Mycoplasma Test | Negative |
| Sterility Test | Negative |
| Endotoxin Test | < 0.01 EU per μg |
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution;
- 2-8°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) at 5 μg/mL (100 μL/well) can bind Human Laminin 221 Protein, premium grade (Cat. No. LA2-H5269) with a linear range of 0.2-20 ng/mL (QC tested).
Protocol
Immobilized Human Laminin 221 Protein, premium grade (Cat. No. LA2-H5269) at 5 μg/mL (100 μL/well) can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) immobilized on CM5 Chip can bind Human Laminin 221 Protein, premium grade (Cat. No. LA2-H5269) with an affinity constant of 49.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
