膜杰作
Star Staining
| ID | Components | Size |
| RAS104-C01 | Pre-coated SARS-CoV-2 Spike RBD(BA.2) Microplate | 1 plate |
| RAS104-C02 | Anti-SARS-CoV-2 Antibody (Control, IgA) | 100 μL |
| RAS104-C03 | HRP-Goat anti-Human IgA | 50 μL |
| RAS104-C04 | 10xWashing Buffer | 50 mL |
| RAS104-C05 | Dilution Buffer | 50 mL |
| RAS104-C06 | Substrate Solution | 12 mL |
| RAS104-C07 | Stop Solution | 7 mL |
背景（Background）
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective Assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
应用说明（Application）
This kit is developed for titer detection of Anti-SARS-CoV-2 (BA.2) Antibody IgA (Spike RBD) in human serum.
It is for research use only.
存储 & 运输（Storage & Shipping）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit is used to measure the levels of Anti-SARS-CoV-2 antibody IgA by employing an indirect ELISA format. The microplate in the kit has been pre-coated with SARS-CoV-2 Spike RBD(BA.2). First add the diluted samples to the plate and wash the wells after incubation. Afterwards add HRP-Goat anti-Human IgA to the plate and wash the wells after incubation. Lastly the substrate is loaded into the wells and color develops in proportion to the amount of antibody. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm. The OD Value reflects the amount of antibody bound.
Your experiment will include 4 simple steps:
a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Goat anti-Human IgA to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
典型数据-Typical Data Please refer to Ds document for the assay protocol.
Detection of Anti-SARS-CoV-2 Antibody, Human IgA titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD(BA.2) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgA in 1:200 human serum. Detection was performed using HRP-Conjugated Anti-human IgA antibody with sensitivity of 48 ng/mL (QC tested).
