膜杰作
Star Staining
分子别名（Synonym）
IL21R,CD360,NILR
表达区间及表达系统（Source）
Cynomolgus IL-21 R, Fc Tag (ILR-C5259) is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Glu 232 (Accession # XP_005591578.2).
Predicted N-terminus: Cys 20
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.0 kDa. The protein migrates as 60-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Cynomolgus IL-21 R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Cynomolgus IL-21 R, Fc Tag (Cat. No. ILR-C5259) is more than 85% and the molecular weight of this protein is around 115-140 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Cynomolgus IL-21, His Tag (Cat. No. IL1-C52H9) at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-21 R, Fc Tag (Cat. No. ILR-C5259) with a linear range of 1-20 ng/mL (QC tested).
Protocol
背景（Background）
Interleukin-21 receptor (IL21R) is also known as CD antigen CD360, Novel interleukin receptor (NILR), is a receptor for interleukin-21 and belongs to the type I cytokine receptor family or type 4 subfamily. IL21R contains two fibronectin type-III domains. IL21R is selectively expressed in lymphoid tissues and most highly expressed in thymus and spleen. IL21R transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells.
