膜杰作
Star Staining
分子别名（Synonym）
ADORA2A,adenosine A2a receptor,A2aR, ADORA2, RDC8
表达区间及表达系统（Source）
Human ADORA2A Full Length Protein-VLP (ADA-H52P4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ser 412 (Accession # P29274-1).
Predicted N-terminus: Met 1
蛋白结构（Molecular Characterization）
The protein has a calculated MW of 47.9 kDa | 56.1 kDa.
Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.
The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
活性（Bioactivity）-ELISA
Immobilized Human ADORA2A Full Length Protein-VLP (Cat. No. ADA-H52P4) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human ADORA2A antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
均一性（Identity）-DLS
The mean peak Radius of VLP is 55-70 nm with more than 95% intensity as determined by dynamic light scattering (DLS).
背景（Background）
Adenosine Receptor Subtype A2a (ADORA2A) is a G-protein coupled adenosine receptor encoded by the ADORA2A gene. It is mainly involved in pathophysiological processes including neuroinflammation, synaptic plasticity in glutamatergic synapses and neurogenesis.
