膜杰作
Star Staining
抗体来源（Source）
Anti-SARS-CoV-2 Nucleocapsid Antibody-A5134, Human IgG1 is isolated from a Nucleocapsid protein infected Human and is recombinantly produced from human 293 cells (HEK293).
克隆号（Clone）
A5134
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
特异性（Specificity）
This product is a specific antibody specifically reacts with Nucleocapsid protein.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-5 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Anti-SARS-CoV-2 Nucleocapsid Antibody-A5134, Human IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Anti-SARS-CoV-2 Nucleocapsid Antibody-A5134, Human IgG1 (Cat. No. NUN-M507) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody-A5134, Human IgG1 (Cat. No. NUN-M507) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
背景（Background）
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
