膜杰作
Star Staining
分子别名（Synonym）
Interleukin-24,IL-24,Melanoma differentiation-associated gene 7 protein,MDA-7,Suppression of tumorigenicity 16 protein,IL24,MDA7,ST16
表达区间及表达系统（Source）
Human IL-24 Protein, His Tag (IL4-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 52 - Leu 206 (Accession # Q13007-1).
Predicted N-terminus: Gln 52
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 20.0 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL-24 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-BLI
Loaded Human IL-22 R alpha 1 Protein, Fc Tag (Cat. No. IL1-H5258) on Protein A Biosensor, can bind Human IL-24 Protein, His Tag (Cat. No. IL4-H52H4) with an affinity constant of 1.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
背景（Background）
Interleukin-24 (IL-24, also known as melanoma differentiation-associated gene-7) belongs to the family of IL-10-related cytokines, which includes IL-10, -19, -20, -22, -24 and -26. Interleukin 24 signals through two heterodimeric receptors: IL-20R1/IL-20R2 and IL-22R1/IL-20R2. Upon binding to its receptors, IL-24 induces rapid activation of Stat-1 and Stat-3 transcription factors, which appear to play a role in cell survival and proliferation . Structurally and functionally, IL-24 is highly conserved across species.IL-24 has been shown to possess cancer-specific apoptosis-inducing activity, and has therefore been suggested as a potential anti-cancer agent.
