膜杰作
Star Staining
& Cat.No. | Items | Details |
| Detection Method | Chemiluminescence |
| Product Type | Magnetic Beads (Protein A) |
| Quantity Size | 10mg / 100mg |
| Physical Appearance | lyophilized powder mixture |
| Particle size | 2.8µm |
| Beads Surface | Hydrophilic |
| Amount of Coupled Protein | About 755 pmol (20 μg) Protein A/mg Beads |
| Binding Capacity | 15-25ug Human antibody or Human IgG Fc tag proteins/mg beads |
| Emission Wavelength | Measured relative light units (RLU) at 430 nm |
背景（Background）
The Protein A-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of Diamond Protein A. The beads can be used to capture the antibodies in Chemiluminescence procedures.The Diamound Protein A is a 26.5 kDa surface protein, it binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family.The Protein A-coupled Magnetic Beads is easy to capture the most antibodies, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
应用说明（Application）
The Protein A-coupled Magnetic Beads is used to capture the most antibodies, it can combination with Acridinium ester markers in chemiluminescence technology, The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, this allows detection of antigen and antibody binding or antibody screening.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
活性（Bioactivity）-MPCLIA
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Protein A-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A001) (10 μg beads/Test). Detection was performed with sensitivity of 3.125 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Protein A-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A001) is stable at 37°C for 10 days.
The MPCLIA assay shows that Protein A-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A001) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Protein A-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A001) is stable at 37°C for 3 days.
