膜杰作
Star Staining
| ID | Components | Size |
| RAS134-C01 | Pre-coated SARS-CoV-2 Spike RBD (BA.5) Microplate | 1 plate |
| RAS134-C02 | SARS-CoV-2 Antibody Positive Control | 100 μL |
| RAS134-C03 | SARS-CoV-2 Antibody Negative Control | 100 μL |
| RAS134-C04 | HRP-Anti-Human IgG | 100 μL |
| RAS134-C05 | 10xWashing Buffer | 50 mL |
| RAS134-C06 | Dilution Buffer | 50 mL |
| RAS134-C07 | Substrate Solution | 12 mL |
| RAS134-C08 | Stop Solution | 7 mL |
背景（Background）
According to the data from GISAID, until 25th July, BA.5 was accounted for 52% of the sequence library, making it the dominant strain in a new global outbreak. Some research results showed that BA.5 seems to be the most infectious version of SARS-CoV-2 with the R0 value of 18.6 (about 2.79 in wild type). Furthermore, the strong immune escape ability is also a powerful blessing for BA.5 to quickly become the dominant strain. A report from New York showed that the breakthrough infection capacity of BA.5 should be 4.2 times of BA.1. The latest research shows that BA.5 has also a strong immune escape from the serum of BA.1 infected people, which means that the existing "immune wall" in the population may be ineffective against BA.5.To facilitate the mutant-related research, drug trials and vaccine development in clinical reasearch, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.
应用说明（Application）
This Kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (BA.5) Antibody IgG (Spike RBD) in human serum.
It is for research use only.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD (BA.5). The kit consists of Pre-coated SARS-CoV-2 Spike RBD (BA.5) Microplate, an Positive Control, an Negative Control,an HRP-Anti-Human IgG secondary antibody and related buffer.
Your experiment will include 4 simple steps:
a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
