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ClinMax™ Human IL-2 ELISA Kit

For research use only.

产品概述(Product Details)

ClinMax™ Human IL-2 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-2 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human IL-2 in serum, plasma and cell culture supernatants.

It is for research use only.

流程图(Workflow)

IL-2 Workflow

关键信息(Key Features)

AnalyteIL-2
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity5.0 pg/mL
Range6.25 pg/mL-800 pg/mL
Assay Time1.75 hr
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

IL-2 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-2 is less than 5.0pg/mL.

 

验证(Validation)

样本值(Sample Values)

Serum Sample – 240 samples from healthy volunteers were tested for the presence of human IL-2 concentration in the assay. 184 samples were detectable. No medical histories were available for the donors used in this study.

IL-2 SAMPLE VALUES

基质效应(Matrix Effect)

6 types of materials were tested to observe if there were matrix effect (interference). If the concentration of bilirubin (simulated jaundice) is less than 20 mg/dL, the concentration of hemoglobin (simulated hemolysis) is less than 3500 mg/dL, the concentration of triglyceride (simulated lipid blood) is less than 2.0 g/L, Heparin concentration is less than 40U/mL, EDTA concentration is less than 4 mg/mL, and Sodium Citrate Plasma concentration is less than 40 mg/mL, testing results will not be affected.

IL-2 MATRIX EFFECT

稀释线性(Dilution Linearity)

High concentrations of human IL-2 serum samples were diluted with 1:2, 1:4, and 1:8 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of IL-2 was 97.38%.

IL-2 DILUTION LINEARITY

批内差异(Intra-Assay Statistics)

10 replicates of each of 3 samples containing different IL-2 concentrations were tested in one assay. Acceptable criteria: CV<10%.

IL-2 INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

3 samples containing different concentrations of IL-2 were tested in the independent assays. Acceptable criteria: CV<15%.

IL-2 INTER-ASSAY STATISTICS

回收率(Recovery)

IL-2 was spiked into 5 human serum samples, and then analyzed. The average recovery of IL-2 for serum samples is 97.58%.

IL-2 RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CRB008-C01Pre-coated Anti-IL-2 Antibody Microplate1 plate
CRB008-C02Human IL-2 Standard25 μg×2
CRB008-C03Biotin-Anti-IL-2 Antibody Con. Solution100 μL
CRB008-C04Biotin-Antibody Dilution Buffer8 mL
CRB008-C05Streptavidin-HRP Con. Solution500 μL
CRB008-C06Streptavidin-HRP Dilution Buffer15 mL
CRB008-C0720× Washing Buffer50 mL
CRB008-C081× Dilution Buffer15 mL×2
CRB008-C09Substrate Solution12 mL
CRB008-C10Stop Solution6 mL

 

前沿进展

 
 
货号/价格
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+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
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order.cn@acrobiosystems.com
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IL-2靶点信息
英文全称:Interleukin-2
中文全称:白细胞介素-2
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:35详情
最高研发阶段:批准上市
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