膜杰作
Star Staining
抗体来源（Source）
Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) is isolated from a Spike RBD infected Mouse and is recombinantly produced from human 293 cells (HEK293).
克隆号（Clone）
10B1A5
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant SARS-CoV-2 Spike Trimer Protein (BA.4/Omicron) erived from human 293 cells.
特异性（Specificity）
This product is a specific antibody specifically reacts with Spike RBD.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.5-500 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) is more than 95% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) with a linear range of 0.5-63 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.2.12.1/Omicron) (Cat. No. SPD-C522q) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) (Cat. No. SPD-C522r) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 Spike RBD, His Tag (BA.4&BA.5/Omicron) (Cat. No. SPD-C522r) with an affinity constant of 15.5 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 Spike RBD, His Tag (BA.2.12.1/Omicron) (Cat. No. SPD-C522q) with an affinity constant of 11.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Anti-SARS-CoV-2 Spike RBD Antibody, Human IgG1 (10B1A5) (BA.2&BA.4&BA.5/Omicron Specific) (Cat. No. SPD-M541) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) with an affinity constant of 11.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
