膜杰作
Star Staining
表达区间及表达系统（Source）
Biotinylated Human HLA-A*02:01&B2M&RHAMM-R3 (ILSLELMKL) Complex Protein (HLR-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & ILSLELMKL peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & ILSLELMKL).
Predicted N-terminus: Gly 25 & Ile 21
Request for sequence
蛋白结构（Molecular Characterization）
Biotinylated Human HLA-A*02:01&B2M&RHAMM-R3 (ILSLELMKL) Complex Protein is produced by co-expression of HLA and B2M loaded with RHAMM-R3 peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-44 kDa and 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Biotinylated Human HLA-A*02:01&B2M&RHAMM-R3 (ILSLELMKL) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human HLA-A*02:01&B2M&RHAMM-R3 (ILSLELMKL) Complex Protein (Cat. No. HLR-H82E6) is more than 90% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human HLA-A*02:01&B2M&RHAMM-R3 (ILSLELMKL) Complex Protein (Cat. No. HLR-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
背景（Background）
The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with a higher risk of breast cancer. Alternatively, spliced transcript variants encoding different isoforms have been noted for this gene. The Human HLA-A*0201 RHAMM-R3 (ILSLELMKL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and ILSLELMKL peptide of the RHAMM-R3.
