膜杰作
Star Staining
分子别名（Synonym）
IL21R,CD360,NILR
表达区间及表达系统（Source）
Biotinylated Mouse IL-21 R Protein, His,Avitag (ILR-M82E3) is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Pro 236 (Accession # Q9JHX3-1).
Predicted N-terminus: Cys 20
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.5 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Mouse IL-21 R Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human IL-21, Fc Tag (Cat. No. IL1-H5253) at 5 μg/mL (100 μL/well) can bind Mouse IL-21 R Protein, His,Avitag (Cat. No. ILR-M82E3) with a linear range of 1-39 ng/mL (QC tested).
Protocol
背景（Background）
Interleukin-21 receptor (IL21R) is also known as CD antigen CD360, Novel interleukin receptor (NILR), is a receptor for interleukin-21 and belongs to the type I cytokine receptor family or type 4 subfamily. IL21R contains two fibronectin type-III domains. IL21R is selectively expressed in lymphoid tissues and most highly expressed in thymus and spleen. IL21R transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells.
