描述(Description)
HEK293/Human ASGR1 Stable Cell Line
应用说明(Application)
Binding assay by FACS and cell based ELISA.
生长特性(Growth Properties)
Adherent
筛选标记(Selection Marker)
Hygromycin B (100 μg/mL)
培养基(Culture Medium)
DMEM medium + 10% FBS
冻存液(Freeze Medium)
10% DMSO + 90% FBS
装量(Quantity)
1 vial contains at least 5x10^6 cells in 1 ml of 10% DMSO in FBS.
存储(Storage)
Frozen in liquid nitrogen.
支原体检测(Mycoplasma Testing)
Negative
无菌检测(Sterility Testing)
Negative
使用说明(Instructions for Use)
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Fig. FACS analysis of ASGR1 on HEK293/Human ASGR1 Stable Cell Line.
FACS assay shows that Anti-ASGR1 Antibody can bind to HEK293/Human ASGR1 Stable Cell Line. HEK293/Human ASGR1 Stable Cell Line was red line, Negative control HEK293 cells was grey line (QC tested).
Protocol
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背景(Background)
ASGPR, a transmembrane C-type lectin, recognizes a wide variety of ligands that contain either terminal galactose (Gal) or N-acetylgalactosamine (GalNAc) residues and has been identified as a highly selective receptor on hepatocytes. The ASGR is composed of both a major (ASGR1) and minor subunit (ASGR2). ASGR1 has been shown to be efficiently targeted to the plasma membrane and to undergo constitutive endocytosis and recycling and found mainly expressed in the human liver.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.