膜杰作
Star Staining
抗体来源（Source）
Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) is isolated from a Spike RBD infected Mouse and is recombinantly produced from human 293 cells (HEK293).
克隆号（Clone）
2G7F5
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant SARS-CoV-2 Spike Trimer Protein (BA.4/Omicron) erived from human 293 cells.
特异性（Specificity）
This product can broadly reacts with SARS-CoV-2 Spike protein of WT and variant.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-100 ng/mL |
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) (Cat. No. SPD-M540) is more than 90% and the molecular weight of this protein is around 130-150 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522e) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) (MALS verified) (Cat. No. SPD-M540) with a linear range of 0.1-6 ng/mL (QC tested).
Protocol
Immobilized Different SARS-CoV-2 Spike RBD, His Tag at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) (MALS verified) (Cat. No. SPD-M540) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
背景（Background）
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
