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 >  Antibody>Spike RBD >SPD-M540

Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) (MALS verified)

抗体来源(Source)

Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) is isolated from a Spike RBD infected Mouse and is recombinantly produced from human 293 cells (HEK293).

克隆号(Clone)

2G7F5

亚型(Isotype)

Human IgG1 | Human Kappa

偶联(Conjugate)

Unconjugated

抗体类型(Antibody Type)

Recombinant Monoclonal

种属反应性(Reactivity)

Virus

免疫原(Immunogen)

Recombinant SARS-CoV-2 Spike Trimer Protein (BA.4/Omicron) erived from human 293 cells.

特异性(Specificity)

This product can broadly reacts with SARS-CoV-2 Spike protein of WT and variant.

应用(Application)

ApplicationRecommended Usage
ELISA0.1-100 ng/mL

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

纯化(Purification)

Protein A purified/ Protein G purified

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Spike RBD SDS-PAGE

Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Spike RBD SEC-MALS

The purity of Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) (Cat. No. SPD-M540) is more than 90% and the molecular weight of this protein is around 130-150 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Spike RBD ELISA

Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522e) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) (MALS verified) (Cat. No. SPD-M540) with a linear range of 0.1-6 ng/mL (QC tested).

Protocol

Spike RBD ELISA

Immobilized Different SARS-CoV-2 Spike RBD, His Tag at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike Broadly Antibody, Human IgG1 (2G7F5) (MALS verified) (Cat. No. SPD-M540) with a linear range of 0.1-3 ng/mL (Routinely tested).

Protocol

 

背景(Background)

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

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