膜杰作
Star Staining
产品概述（Product Details）
ClinMax™ Human HGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human HGF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M13 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant human HGF in serum, plasma and cell culture supernatants.
It is for research use only.
流程图（Workflow）
关键信息（Key Features）
| Analyte | HGF |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 40 pg/mL |
| Range | 62.5 pg/mL-8000 pg/mL |
| Assay Time | 1 hr 45 min |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
存储（Storage）
Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of HGF is less than 40.0 pg/mL.
组分（Materials Provided）
| ID | Components | Size |
| CRB012-C01 | Pre-coated Anti-HGF Antibody Microplate | 1 plate |
| CRB012-C02 | Human HGF Standard | 20 μg×2 |
| CRB012-C03 | Biotin-Anti-HGF Antibody Con. Solution | 100 μL |
| CRB012-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CRB012-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CRB012-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CRB012-C07 | 20× Washing Buffer | 50 mL |
| CRB012-C08 | Sample Dilution Buffer | 15 mL×2 |
| CRB012-C09 | Substrate Solution | 12 mL |
| CRB012-C10 | Stop Solution | 6 mL |
