膜杰作
Star Staining
抗体来源（Source）
Biotinylated Anti-Adalimumab Antibody (22E7A4A2) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
22E7A4A2
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Kappa
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Adalimumab.
特异性（Specificity）
Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.4-12.5 ng/mL |
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Anti-Adalimumab Antibody (22E7A4A2) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (Cat. No. ADB-BY17c) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Adalimumab at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (Cat. No. ADB-BY17c) with a linear range of 0.4-3 ng/mL (QC tested).
Protocol
背景（Background）
Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant D technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.
