登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Claudin-18.1 >CL1-H52P3

Human Claudin-18.1 Full Length Protein (VLP)

分子别名(Synonym)

Claudin-18.1,CLDN18,Claudin-18

表达区间及表达系统(Source)

Human Claudin-18.1 Full Length Protein (VLP) (CL1-H52P3) is expressed from human 293 cells (HEK293). It contains AA Met 1- Val 261 (Accession # P56856-1).

Predicted N-terminus: Met 1

蛋白结构(Molecular Characterization)

The protein has a calculated MW of 29.7 kDa | 56.1 kDa.

Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

制剂(Formulation)

The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 12 months under sterile conditions.
*The isotype control of empty/mock VLP (Cat. No. VLP-N5213) is sold separately and not included in protein, you can follow this link for product information.
 

活性(Bioactivity)-ELISA

Claudin-18.1 ELISA

Immobilized Human Claudin-18.1 Full Length Protein (VLP) (Cat. No. CL1-H52P3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Cludin18.1 antibody, Mouse IgG with a linear range of 2-78 ng/mL (QC tested).

Protocol

 

均一性(Identity)-DLS

Claudin-18.1 DLS

The mean peak Radius of VLP is 75-95 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

 

背景(Background)

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010]

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Claudin-18.1靶点信息
英文全称:Claudin-18 splice variant 2
中文全称:密蛋白-18剪接变体2
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:61详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定