膜杰作
Star Staining
& Cat.No. | Product | Size | Amount |
| ActiveMax® Streptavidin μBeads, premium grade (for cells) | 10 mg | 1 × 10⁸ beads |
| ActiveMax® Streptavidin μBeads, premium grade (for cells) | 100 mg (10 mg × 10) | 1 × 10⁹ beads |
背景（Background）
ActiveMax® Streptavidin μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm with streptavidin on its surface. Streptavidin is a tetrameric protein providing 4 high-affinity biotin binding sites, which make it possible to fast prepare and customize your own sterile protein/antibody coupled beads for cell based assay, cell sorting, cell therapy process development and manufacturing use.
表达区间及表达系统（Source）
ActiveMax® Streptavidin coupled-μbeads, premium grade (for cells) are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.
应用说明（Application）
ActiveMax® Streptavidin coupled-μbeads, premium grade (for cells)can be used for coupling of any biotinylated proteins or antibodies, and are ideal for numerous applications as follows:
When coupled with biotinylated CAR target proteins, it can be used to stimulate or isolate CAR-T cells or UCAR-T cells.
When coupled with biotinylated antibodies, it can be used to isolate antigen specific cells, including immune cells, tumor cells, etc.
When coupled with biotinylated drug targets, it can be used for screening target specific antibodies by FACS.
It can also be used for other cell based experiments that you need.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 6 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.002 EU per μg by the LAL method.
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data Please refer to DS document for the assay protocol.
ActiveMax Streptavidin coupled μbeads, premium grade coupled with protein. 40ug of the biotinylated human BCMA protein (Cat. No. BCA-H82E4) was added to 1mg of ActiveMax Streptavidin coupled μbeads, premium grade (for cells) (Cat. No. MBS-C009), mix well and incubate for 60 mins at room temperature. And then the coupled μbeads were fluorescently stained using PE labeled anti-human BCMA antibody and analyzed by flow cytometry.
ActiveMax Streptavidin coupled μbeads, premium grade coupled with human BCMA protein. The biotinylated human BCMA protein (Cat. No. BCA-H82E4) in serial dose was added to 1mg of ActiveMax Streptavidin coupled μbeads, premium grade (for cells) (Cat. No. MBS-C009), mix well and incubate for 60 mins at room temperature. And then the unbound protein in supernatant measured by Micro BCA Protein Assay Kit was subtracted to calculate the BCMA protein coupled amount on the μbeads (loaded capacity).
ActiveMax Streptavidin coupled μbeads, premium grade coupled with antibody. 50ug of the biotinylated anit-FMC63 Antibody (Cat. No. FM3-BY54) was added to 1mg of ActiveMax Streptavidin coupled μbeads, premium grade (for cells) (Cat. No. MBS-C009), mix well and incubate for 60 mins at room temperature. And then the coupled μbeads were fluorescently stained using PE labeled anti-mouse Fc antibody and analyzed by flow cytometry.
ActiveMax Streptavidin coupled μbeads, premium grade coupled with Antibody. The anit-FMC63 Antibody (Cat. No. FM3-BY54) in serial dose was added to 1mg of ActiveMax Streptavidin coupled μbeads, premium grade (for cells) (Cat. No. MBS-C009), mix well and incubate for 60 mins at room temperature. And then the unbound protein in supernatant measured by Micro BCA Protein Assay Kit was subtracted to calculate the antibody coupled amount on the μbeads (loaded capacity).
