膜杰作
Star Staining
分子别名（Synonym）
75 kDa DNA-pairing protein,ALS6,amyotrophic lateral sclerosis 6,FUS1,fused in sarcoma,fus-like protein,Oncogene FUS,Oncogene TLS,RNA-binding protein FUS,POMP75,Translocated in liposarcoma,Translocated in liposarcoma protein
表达区间及表达系统（Source）
Human FUS Protein, His Tag (FUS-H5144) is expressed from E. coli cells. It contains AA Met 1 - Tyr 526 (Accession # P35637-1).
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a MBP tag at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 97.0 kDa. The protein migrates as 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>85% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human FUS Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human FUS Protein, His Tag (Cat. No. FUS-H5144) at 1 μg/mL (100 μL/well) can bind FUS antibody, Mouse IgG with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
背景（Background）
FUS (R-binding protein FUS) is a D/R-binding protein that plays a role in various cellular processes such as transcription regulation, R splicing, R transport, D repair and damage response. It binds to nascent pre-mRs and acts as a molecular mediator between R polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. It binds also its own pre-mR and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay.
FUS plays a role in D repair mechanisms by promoting D-loop formation and homologous recombination during D double-strand break repair. In neuronal cells, FUS plays crucial roles in dendritic spine formation and stability, R transport, mR stability and synaptic homeostasis.
