膜杰作
Star Staining
产品概述（Product Details）
ClinMax™ Human Granzyme B ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human Granzyme B that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant human Granzyme B in serum, plasma and cell culture supernatants.
It is for research use only.
流程图（Workflow）
关键信息（Key Features）
| Analyte | Granzyme B |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 1 pg/mL |
| Range | 3.906 pg/mL-500 pg/mL |
| Assay Time | 2 hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 μL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of Granzyme B is less than 1 pg/mL.
组分（Materials Provided）
| ID | Components | Size |
| CEA033-C01 | Pre-coated Anti- Granzyme B Antibody Microplate | 1 plate |
| CEA033-C02 | Human Granzyme B Standard | 20 μL |
| CEA033-C03 | Biotin-Anti- Granzyme B Antibody Con. Solution | 100 μL |
| CEA033-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA033-C05 | Streptavidin-HRP Con. Solution | 0.5 mL |
| CEA033-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA033-C07 | 20× Washing Buffer | 50 mL |
| CEA033-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA033-C09 | Substrate Solution | 12 mL |
| CEA033-C10 | Stop Solution | 6 mL |
